It will be sufficient for efficient focussing. Bringing you a world of innovation To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. They further discussed superior sensitivity levels in relation to both a colloidal Coomassie protocol with CBB G in phosphoric acid, ammonium sulfate and methanol, and an acidic silver staining Silver Stain Plus Kit from Amersham Pharmacia Biotechnology. When two strips have the same pH range but differ in length — the ratio of the strip lengths gives the relative focusing power of the longer strip. Additionally, occurrence of microdomains hampers quantitative protein solubilisation and therefore membrane proteomics remain a major challenge.